价格
￥1,680.00
型号
RG011,OD104
品牌
Pickering/皮克林
所在地
天津市 南开区
更新时间
2020-08-26 12:40:04
浏览次数
次
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RG011,OD104,皮克林/Pickering柱后衍生
OPA 用于衍生分析氨基酸是1971年首次提出。图 1 为 OPA 与一级氨基酸的反应方程式。
室温下， OPA （Pickering O120） 在巯基乙醇(Pickering 3710-2000)环境中与一级氨基酸于 1 min 内即可生成具有强荧光的异吲哚衍生产物。OPA 本身不发荧光的特性使其在色谱分离时不形成干扰峰。OPA 只能与经次氯酸钠等氧化剂氧化开环后的二级氨基酸反应，也可与 FMOC － Cl 联用衍生二级氨基酸。OPA 衍生法因样品制备简单、衍生反应迅速、灵敏度高及易于实现自动化操作等优点而成为柱前衍生反相高效液相色谱法中使用广泛的方法。
OPA 衍生法（用于检测一级氨基酸）
试剂： 300 mg OPA （Pickering O120）, 2 g Thiofluor™ (Pickering 3710-2000), 3 mL 30 % Brij® 35 溶于 950 mL OPA稀释液(Pickering OD104)中
反应器： 45 °C, 0.15 mL
衍生试剂流速： 0.3 mL/min
检测器： 荧光检测器
λex= 330 nm, λem=465 nm
相关产品目录
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